Sample Submission

How to Get Started

To request a pricing quote or prepare a sample for sequencing, please create an account on the North Texas Genome Center’s customer portal hosted by Idea Elan. Once you have registered and received a pricing estimate, please review the information below to learn how to prepare your samples for submission.

Sample Submission Forms

The quality of sequencing results depends on the purity and integrity of the template DNA/total RNA. Use a fluorometric-based method for quantification, such as Qubit or PicoGreen to provide accurate quantifications for dsDNA and total RNA. UV spectrophotometric based methods, such as the Nanodrop, measures any nucleotides present in the sample including RNA, dsDNA, ssDNA and free nucleotides, which can be given inaccurate measurement of genomic DNA. Generally, the use of the commercially available DNA/total RNA purification kits to prepare the templates will yield sequence quality DNA/RNA.

To submit samples for processing in the North Texas Genome Center, please complete the sample submission form below for either DNA/total RNA samples or customer-prepared libraries being submitted for library preparation. Send your completed forms to zibiao.guo@uta.edu .

NGS Samples for Library Preparation Submission

Below are the quantity and volumes needed for sample submission depending on which services you request. If you must submit samples in volume or concentration that differs from what is listed below, please contact us at zibiao.guo@uta.edu .

Submit samples in 1.5 ml Lo-bind microfuge tubes, labeled clearly with order number, submission date and sample identity. Please quantify the DNA and RNA concentrations using fluorometry. Sample quantification is required prior to submission.

Library TypesMinimum QuantityA260/280RNA Integrity (RIN)Concentration (per Qubit)Shipment Method
RNA-Seq Samples5 ug>1.8-2.0>7.0~100 ng/uldry ice
mRNA Sequencing5 ug>1.8-2.0>7.0~100 ng/uldry ice
Whole Transcriptome2 ug>1.8-2.0>7.0~100 ng/uldry ice
Illumina Small RNA Seq2 ug>1.8-2.0>7.0~200 ng/uldry ice
ChIP-Seq Samples (DNA should be sheared to 200-600 bp)5 ng for the ChIP DNA sample (50 ng for the input DNA sample)>1.8-2.0dry ice
Exome Sequencing1-5 ug~100 ng/uldry ice
Whole Genome Sequencing2 ug>1.8-2.0>20 ng/uldry ice
16S Metagenomic Samples20 ng>5 ng/uldry ice

Prepared Libraries for Submission

All clients are responsible for their own QC (quantification and fragment analysis) and library pooling prior to submission.

NovaSeq 6000StandardXP Workflow (per lane)
SP/S14nM at 110uL4nM at 25 uL
S24nM at 165uL4nM at 30 uL
S44nM at 325uL4nM at 40uL
iSeqSP/S1, S2, S44nM at 25uL
MiSeqSP/S1, S2, S44nM at 25 uL

Note: If the pool concentration or volume is below the submission requirement listed in the above table, contact zibiao.guo@uta.edu for further instruction and advice.

Questions?

We are here to help! Contact Zibiao Guo, Senior Research Scientist, at zibiao.guo@uta.edu.