How to Get Started
To request a pricing quote or prepare a sample for sequencing, please create an account on the North Texas Genome Center’s customer portal hosted by Idea Elan. Once you have registered and received a pricing estimate, please review the information below to learn how to prepare your samples for submission.
Sample Submission Forms
The quality of sequencing results depends on the purity and integrity of the template DNA/total RNA. Use a fluorometric-based method for quantification, such as Qubit or PicoGreen to provide accurate quantifications for dsDNA and total RNA. UV spectrophotometric based methods, such as the Nanodrop, measures any nucleotides present in the sample including RNA, dsDNA, ssDNA and free nucleotides, which can be given inaccurate measurement of genomic DNA. Generally, the use of the commercially available DNA/total RNA purification kits to prepare the templates will yield sequence quality DNA/RNA.
To submit samples for processing in the North Texas Genome Center, please complete the sample submission form below for either DNA/total RNA samples or customer-prepared libraries being submitted for library preparation. Send your completed forms to zibiao.guo@uta.edu .
NGS Samples for Library Preparation Submission
Below are the quantity and volumes needed for sample submission depending on which services you request. If you must submit samples in volume or concentration that differs from what is listed below, please contact us at zibiao.guo@uta.edu .
Submit samples in 1.5 ml Lo-bind microfuge tubes, labeled clearly with order number, submission date and sample identity. Please quantify the DNA and RNA concentrations using fluorometry. Sample quantification is required prior to submission.
Library Types | Minimum Quantity | A260/280 | RNA Integrity (RIN) | Concentration (per Qubit) | Shipment Method |
RNA-Seq Samples | 5 ug | >1.8-2.0 | >7.0 | ~100 ng/ul | dry ice |
mRNA Sequencing | 5 ug | >1.8-2.0 | >7.0 | ~100 ng/ul | dry ice |
Whole Transcriptome | 2 ug | >1.8-2.0 | >7.0 | ~100 ng/ul | dry ice |
Illumina Small RNA Seq | 2 ug | >1.8-2.0 | >7.0 | ~200 ng/ul | dry ice |
ChIP-Seq Samples (DNA should be sheared to 200-600 bp) | 5 ng for the ChIP DNA sample (50 ng for the input DNA sample) | >1.8-2.0 | dry ice | ||
Exome Sequencing | 1-5 ug | ~100 ng/ul | dry ice | ||
Whole Genome Sequencing | 2 ug | >1.8-2.0 | >20 ng/ul | dry ice | |
16S Metagenomic Samples | 20 ng | >5 ng/ul | dry ice |
Prepared Libraries for Submission
All clients are responsible for their own QC (quantification and fragment analysis) and library pooling prior to submission.
NovaSeq 6000 | Standard | XP Workflow (per lane) |
SP/S1 | 4nM at 110uL | 4nM at 25 uL |
S2 | 4nM at 165uL | 4nM at 30 uL |
S4 | 4nM at 325uL | 4nM at 40uL |
iSeq | SP/S1, S2, S4 | 4nM at 25uL |
MiSeq | SP/S1, S2, S4 | 4nM at 25 uL |
Note: If the pool concentration or volume is below the submission requirement listed in the above table, contact zibiao.guo@uta.edu for further instruction and advice.
Questions?
We are here to help! Contact Zibiao Guo, Senior Research Scientist, at zibiao.guo@uta.edu.