The North Texas Genome Center represents a collaborative venture between multiple universities, medical centers, and corporate partners to establish a center of excellence for genomics and high throughput sequencing.
ABOUT THE CENTER
Housed at the University of Texas at Arlington, in the center of the Dallas-Ft. Worth Metroplex, the North Texas Genome Center utilizes a core of five Illumina NovaSeq-6000 cutting edge genome sequencing instruments which will facilitate a projected sequencing capacity equivalent to 25,000 human genomes per year.
The center is opening to customers in the Spring of 2018, and will eventually transition to UTA’s new 220,000-square-foot Science & Engineering Innovation & Research (SEIR) Building, a world-class research and teaching facility scheduled to open later in 2018.
The center also includes technical and administrative staff, along with multiple instruments that automate the processing of biological samples to facilitate very high throughput capacity at excellent economy and scale.
Together with in house and system-based computational infrastructure, the center is directly linked via high capacity fiber to internet service, which rapidly and efficiently delivers data in real time to customers in a cloud-based computing environment (Illumina BaseSpace), where they may readily download and further analyze their data.
MEET THE CENTER STAFF
Dr. Jon Weidanz, Director
Dr. Jon Weidanz, NTGC Founding Director, is an internationally recognized leader in cancer immunotherapy through his pioneering work to attack and destroy cancer cells by targeting Major Histocompatibility Complexes (MHC) presenting tumor specific peptides. He has founded or co-founded four start-up ventures. Dr. Weidanz has collectively more than 80 peer-reviewed articles, book chapters and published conference proceedings. He was awarded the title of “Distinguished University Professor” from Texas Tech University Health Science Center (TTUHSC) as well as was recognized as an outstanding teacher by being awarded the President’s Excellence in Teaching award and the Chancellor’s Distinguished Teacher award. Dr. Weidanz joined The University of Texas at Arlington (UTA) in January of 2016 as the Associate Vice President for Research, and Professor of Biology. He came to UTA to provide leadership and guidance to develop and expand research across campus in life and health science and to guide new investments in facilities on campus.
Dr. Todd Castoe, Associate Director
Dr. Todd Castoe, Associate Director, is an expert in functional genomics, population genetics, and bioinformatics. With over 80 publications in genomics and bioinformatics, he has broad experience developing novel genomic library preparation techniques and developing innovative bioinformatic approaches and software for analysis of human and non-traditional model species genomic data. Castoe previously spent four years as the Co-director of Computational Genomics and the Associate Director of the Consortium for Comparative Genomics and Genome Sequencing Core Facility at the University of Colorado Anschutz Medical Campus, and has experience running and managing both the molecular and bioinformatics components of a medically-oriented genomics facility. Since 2012 Dr. Castoe has been serving as Assistant Professor & Assistant Curator of Herpetology, Department of Biology, The University of Texas at Arlington.
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Illumina NovaSeq 6000:
The NovaSeq 6000 System provides tremendous scalability and flexibility to accommodate diverse applications and project sizes, while achieving maximum operational efficiency. Runs can include a single flow cell or two flow cells simultaneously. Customers have their choice of 3 flow cell types and can easily adjust output for each sequencing run. With such highly tunable data output, we will be able to run virtually any genome, sequencing method, and scale of project. The NovaSeq 6000 System is compatible with a broad range of Illumina library preparation kits, from transcriptome sequencing to whole-genome sequencing and everything in between. High-quality, high-coverage sequencing offers a comprehensive view of the genome to detect variants accurately, characterize isoforms, and more. With increased throughput and reduced price per sample, the NovaSeq 6000 System is ideal for data-intensive applications
The MiSeq system of automated paired-end reads and up to 15 Gb per run, delivering over 600 bases of sequence data per read. The library prep kits that it uses are optimized for a variety of applications, including targeted gene, small genome, and amplicon sequencing, 16S metagenomics, and more.
The NTGC plans on using the iSeq 100 System to conduct smaller projects and experiments, which will allow us to avoid running small sample batches on high-throughput instruments. Additionally, we can use the iSeq to evaluate libraries before a large-scale run, eliminating the risk of high-cost failures on higher throughput sequencers.
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Sample submission guidelines:
- Currently we can only accept pooled libraries of any type or ultra-pure DNA samples for sequencing.
- When shipping, we strongly encourage pooled libraries be submitted in 1.5ml tubes. DNA samples may be submitted in 1.5ml tubes, PCR strip tubes, or 96-well PCR plates. Seal tubes and plates to prevent leakage or cross-contamination. The NTGC is not responsible for sample leakage during shipment.
- Please label samples clearly. Label 1.5ml tubes on both the side and on top of the tube. For strip tubes, label the first and last tube (please keep samples in the same order as listed on the Sample Submission Form). Label plates on front of plate. Sample IDs must be unique identifiers for each sample. Samples cannot be named generically (e.g. Sample 1, Sample 2, etc.).
- For pooled libraries, please submit 500ul at a concentration of 10nM..
- For DNA samples, please ensure a 260:280 of 1.8-2.0 and a 260:230 of 2.0-2.2. Please submit a minimum of 1ug of DNA in a volume of 50ul.. Input quantity should be obtained by fluorescent assay for the most accurate results.
- Please make sure samples are shipped in the proper storage conditions. For pooled libraries, please ship with dry ice. DNA samples should be shipped with wet ice or ice packs. If dropping samples off in person, samples can be stored on wet ice or ice packs.
- Please fill out completely the Sample Submission Form. Send one completed copy via email to firstname.lastname@example.org. Please print out a second completed copy and submit with your samples. If you have questions when filling out the submission form, please email email@example.comPlease ship samples to:
ATTN: Kelly Sheff
500 UTA Blvd., Suite 226
Arlington, TX 76010
If you are delivering samples in person, please visit us on the 4th floor of the ERB building in lab ERB474.