We provide Next Generation Sequencing (NGS) services using Illumina sequencing technology and automated Eppendorf epMotion liquid handling systems.
Please download our full services and fees description here. To request a pricing quote or instructions to prepare a sample for sequencing, please email ntgcresearch@uta.edu.
We offer a full range of service and applications for next generation sequencing.
DNA Sequencing
DNA sequencing applications for variant discovery, detection and examination of genome regulation are offered as follows:
- Whole genome sequencing
- Exome sequencing
- ChIP sequencing
- Methylation sequencing
RNA Sequencing
RNA sequencing is performed to measure genome-wide gene transcription and assays are designed to measure mRNA, total RNA or miRNA species.
- Coding or mRNA
- Total RNA with ribosomal depletion
- FFPE
- Small RNA
Metagenomics
- 16S ribosomal profiling
- Shotgun whole genome metagenomic sequencing
Single-Cell Sequencing
Single-Cell Profiling using a 10x genomics platform to perform single cell RNAseq and other single cell applications.
Equipment
Our facility is equipped with state-of-the-art instrumentation for sequencing.
Illumina NovaSeq 6000
The Illumina NovaSeq 6000 is a high-end sequencer designed to provide some of the highest throughput in the market. It has the capacity to run up to two flow cells at a time, with 2 flow cell options to choose from. The highest throughput (S2) can process up to 3.3 billion reads and generate up to 1000 Gb of data (with 150 bp read lengths). The system is compatible with Illumina library preparation kits and can support virtually any sequencing application- whole genome sequencing, whole transcriptomes, whole exomes, metagenomics, and more.
Illumina MiSeq
The Illumina MiSeq System offers capabilities for small whole-genome sequencing, amplicon sequencing, gene expression profiling, small RNA analysis and 16s metagenomic sequencing in a compact instrument. Using a high throughput kit, MiSeq can produce as much as 15 Gb of output from 50 million paired end reads (at a read length of 300 bp).
iSeq 100
The iSeq 100 Sequencing System makes next generation sequencing easier and more affordable than ever. Designed for simplicity, it allows labs of all sizes to sequence DNA and RNA at the push of a button. The iSeq 100 System is ideal for small whole-genome sequencing (eg, bacteria, viruses, plasmids), targeted sequencing of a set of genes or gene regions, gene expression analysis, or 16S metagenomics.
Questions?
We are here to help! Please contact us!